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. 2005 Sep;73(9):6138–6142. doi: 10.1128/IAI.73.9.6138-6142.2005

FIG. 1.

FIG. 1.

B. burgdorferi outer membrane proteins that bind to CR3 affinity columns. Images of silver-stained gels (12% SDS-PAGE) are shown. Left-hand side: whole borrelia and borrelia outer membranes (O.M.). Right-hand side: fractions 1, 2, and 3 (elution with 1 M KCl) from each of the affinity columns A (CR3-Sepharose), B (CR3-Sepharose with bound iC3b), and C (Sepharose without CR3). The background staining observed in the area from 55 to 65 kDa across the whole gel is nonspecific and unrelated to the proteins contained in the samples loaded.