TABLE 2.
M. leprae-specific candidate antigensa
| Gene accession no. | Functional classification | Protein type | Length (bp) | Size (kDa) | Murine and human RT-PCR | % Identityb with:
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M. bovis AF2122/97 (blastp)d | M. tuberculosis H37Rv (blastp)d | M. paratuberculosis K10 (blastp)e | M. microti# (tblastn)d | M. marinum# (tblastn)d | M. avium# (tblastn)f | M. ulcerans# (tblastn)g | M. smegmatis MC2# (tblastn)f | ||||||
| ML0007 | II.C.4 | Conserved membrane | 912 | 32 | + | 53 (Mb0007) | 56 (Rv0007) | 53 (MAP0007) | 48 | 59 | 59 | 56 | 38 |
| ML0126 | VI | Hypothetical | 822 | 31 | + | <30 | <30 | <30 | <30 | <30 | <30 | 67 | <30 |
| ML0369 | VI | Hypothetical | 357 | 13 | + | <30 | <30 | 38 (MAP4250c) | <30 | 33 | 39 | 37 | 33 |
| ML0573 | VI | Hypothetical | 276 | 10 | + | <30 | <30 | <30 | <30 | <30 | <30 | <30 | <30 |
| ML0574 | VI | Hypothetical | 318 | 11 | + | <30 | <30 | <30 | <30 | <30 | <30 | <30 | <30 |
| ML0575 | VI | Hypothetical | 219 | 7 | + | <30 | <30 | <30 | <30 | <30 | <30 | <30 | <30 |
| ML0576 | VI | Hypothetical | 231 | 8 | + | <30 | <30 | <30 | <30 | <30 | <30 | <30 | <30 |
| ML0840 | VI | Hypothetical | 1,305 | 48 | + | <30 | <30 | 59 (MAP2122) | <30 | <30 | 64 | <30 | 47 |
| ML0927 | VI | Hypothetical | 309 | 11 | + | <30 | <30 | 36 (MAP1963c) | <30 | 36 | 35 | 33 | <30 |
| ML1601 | VI | Hypothetical | 366 | 13 | + | <30 | <30 | 33 (MAP3249) | <30 | <30 | 33 | <30 | <30 |
| ML1602 | VI | Hypothetical | 321 | 11 | + | <30 | <30 | <30 | <30 | <30 | <30 | <30 | <30 |
| ML1603 | VI | Hypothetical | 261 | 9 | + | <30 | <30 | <30 | <30 | <30 | <30 | <30 | <30 |
| ML1604 | VI | Hypothetical | 390 | 14 | + | <30 | <30 | <30 | <30 | <30 | <30 | <30 | <30 |
| ML1788 | VI | Very hypothetical | 465 | 17 | + | <30 | <30 | <30 | <30 | <30 | <30 | <30 | <30 |
| ML1989 | VI | Hypothetical | 351 | 13 | + | <30 | <30 | <30 | <30 | <30 | <30 | <30 | <30 |
| ML1990 | II.C.5 | Putative integral membrane | 243 | 9 | + | <30 | <30 | <30 | <30 | <30 | <30 | <30 | <30 |
| ML2283 | VI | Hypothetical | 315 | 12 | + | <30 | <30 | <30 | <30 | <30 | <30 | <30 | <30 |
| ML2567 | VI | Hypothetical | 480 | 17 | + | <30 | <30 | <30 | <30 | <30 | <30 | <30 | <30 |
| ML2649 | IV | Hypotheticalc | 765 | 28 | + | 60 (Mb0270c) | 60 (Rv0264) | 58 (MAP3709c) | 60 | 60 | 63 | 59 | 58 |
a
Annotations for gene accession number, functional classification, and protein are according to the Sanger database. Functional classifications II.C.4, conserved membrane proteins; VI, unknown; II.C.5, other membrane proteins; IV, other.
b
Blast reports were performed December 2004; blastp, protein versus protein; tblastn, protein versus translated DNA. #, Unfinished genomes.
c
According to Leproma (http://genolist.pasteur.fr/Leproma/), ML2649 is a putative oligosaccharide deacetylase.