FIG. 1.

Trial profile demonstrates enrollment numbers, HLA-A2 supertype determination, and quantity of specimens available for immunologic assays. A portion of sample PBMC did not survive the freeze process and were noted to be dead upon thaw. Unresponsive cells appeared intact but did not release IFN-γ in response to positive stimulant (anti-CD3/anti-CD28 beads). Reasons for not performing an immunologic assay included utilization of cells in alternate experiments, cell loss during transit, collection of insufficient cell numbers, and death or loss of follow-up of study participants.