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. 2005 Sep;73(9):5883–5891. doi: 10.1128/IAI.73.9.5883-5891.2005

FIG. 1.

FIG. 1.

Generation and expression of TRAP recombinant proteins. (A) Schematic drawing of P. berghei TRAP indicating the various domains and the design of the construct for the generation of recombinant TRAP proteins. (B) Western blot showing TRAP recombinant proteins probed using an anti-A-domain antibody (lanes 1, 2, 5, and 6) or anti-TRAP (lanes 3 and 4). Lane 1, 5 to 10 μg of GST-TRAP A-domain fusion protein; lane 2, 1 to 2 μg of TRAP A-domain after thrombin cleavage; lane 3, 5 to 10 μg of GST-TRAP repeat fusion protein; lane 4, 1 to 2 μg of TRAP repeat after thrombin cleavage; lane 5, 5 to 10 μg of GST-TRAP/PAdoT (mutant of TRAP A-domain) fusion protein; lane 6, 1 to 2 μg of TRAP/PAdoT protein after thrombin cleavage.