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. 2005 Sep;73(9):5883–5891. doi: 10.1128/IAI.73.9.5883-5891.2005

FIG. 2.

FIG. 2.

Binding of purified recombinant TRAP constructs with HepG2 cells. All the recombinant proteins were cleaved from GST using the thrombin cleavage site. (A) Binding of various concentrations of the purified recombinant TRAP A-domain and GST (as a control) to 1 × 106 HepG2 cells. (B) Binding of 1 μg recombinant TRAP A-domain in the presence of various concentrations of MgCl2, MgCl2 plus 10 mM EDTA, or MgCl2 plus 10 mM EGTA to 1 × 106 HepG2 cells. (C) Binding of 1 μg of the purified recombinant TRAP A-domain to HepG2 cells in the presence of MnCl2, MnCl2 plus 10 mM EDTA, or MnCl2 plus 10 mM EGTA to 1 × 106 HepG2 cells. (D) Binding of various concentrations of the recombinant TRAP repeat protein to 1 × 106 HepG2 cells. Data are averages ± standard deviations from three experiments.