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. 2005 Sep;73(9):5883–5891. doi: 10.1128/IAI.73.9.5883-5891.2005

FIG. 4.

FIG. 4.

Immunoprecipitation of hepatocyte membrane proteins (1 mg) using 100 μg of the purified recombinant TRAP A-domain and GST protein (control) coupled to a protein A column with 50 μg of anti-A-domain and anti-GST antibodies. (A) Silver-stained 4 to 20% gradient gel showing protein eluted from the purified TRAP A-domain recombinant protein column coupled to protein A with anti-A-domain antibodies (lane 1) and no protein eluted from a GST protein column coupled to protein A with anti-GST antibodies (lane 2). (B) Immunoprecipitation of 500 μg of sporozoite soluble protein bound to 100 μg fetuin-A coupled to a protein A column and 50 μg of anti-fetuin A antibodies. Western blotting was performed using anti-TRAP antibodies at a 1:1,000 dilution. TRAP elution from the soluble sporozoite fraction is shown. Lane 1, sporozoite soluble fraction; lane 2, elute 1 after immunoprecipitation; lane 3, elute 2 after immunoprecipitation; lane 4, CS in the soluble fraction of sporozoites before immunoprecipitation; lane 5, no CS detection after immunoprecipitation. For lanes 4 and 5, Western blotting was performed using a 1:1,000 dilution of 3D11 (anti-CS antibodies).