FIG. 10.

Anti-LcrV antibody promotes phagocytosis by PMNs. Glycogen-elicited PMNs from C57BL/6 mice were infected in suspension at an MOI of 3 with Y. pestis KIM5 pVGFP in the presence of 40 μg/ml anti-LcrV antibody (αV) or PBS (No Ab). Phagocytosis was assayed after 1 h as described in the legend to Fig. 5. Numbers of extracellular and intracellular yersiniae were counted in 20 cells in 20 fields. Filled bars, intracellular yersiniae; open bars, extracellular yersiniae. Error bars show ±1 SD from the mean numbers per PMN. The data for αV treatment differed significantly from those for PBS at a P value of <0.0001.