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Anti-Yersinia antibody also reduces the delivery of YopH and YopE. J774A.1 cells in six-well cluster dishes were infected at an MOI of 10 with Y. pestis KIM5(pGFP) in the presence of 40 μg/ml anti-LcrV or anti-Yersinia antibody or of PBS. Delivery of YopH and YopE to the soluble J774A.1 cellular fraction was assayed by immunoblotting. Lanes: PBS, no antibody; αYersinia, anti-Yersinia antibody; α-V, anti-LcrV antibody.
