Fig. 2.

The changes in CFA with the occurrence and development of AS. (A) Masson, Sirius Red, and H&E staining images of the vascular wall at week 10. (B) Masson, Sirius Red, and H&E staining images of the vascular wall at week 20. (C) Masson, Sirius Red, and H&E staining images of the vascular wall at week 30. (D) Statistical analysis of the Col fiber orientation distribution in the innermost layer of media at the 3 stages, showing that CFA in the innermost layer of media gradually becomes random from being oriented (n = 100). The staining images at each time point are from the same mouse. A total of 3 mice were used in this experiment, and the angle analysis statistics are derived from the H&E images.