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MtsR directly binds to the promoter region of shr. (A) Schematic presentation of the sia operon's promoter/operator region (Pshr). The positions of the putative −35 and −10 regions of shr, the sia operon's first gene, are indicated. (B). The EMSA was done with the 32P-labeled promoter fragment and purified MtsR-His protein in a range of concentrations from 0 to 22.5 ng as indicated above the lanes. The DNA-protein complexes C1 and C2 are indicated.
