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ZE491 is attenuated in the zebrafish infection model. GAS cells harvested at the logarithmic growth phase were injected into groups of zebrafish (Danio rerio) (5 × 105 cells per fish) either intramuscularly (IM) or intraperitoneally (IP) as previously described (38). The average number of dead fish at 48 h postinjection is shown for each infection route; error bars represent the standard error of the mean (n = 5).
