TABLE 1.
Oligonucleotide primers used in this study
| Name | Sequence | Location |
|---|---|---|
| sia GS F | 5′ GGCGCGGATCCTCAGCTCTAGTATTAACTATC 3′ | Pshr |
| sia GS R | 5′ GGCGCGGATTCCATTTTCAATCAACTTTCT 3′ | shr |
| SRAL | 5′ GCGTTCAGGAGGTCTAGCTC 3′ | recA |
| SRAR | 5′ CTGATGCTACTGCCATAGCAG 3′ | recA |
| orfX-delA | 5′ CCCGAATTCTGATTGAACTTCACATCTAA 3′ | shr |
| orfX-delS | 5′ CCCGAATTCAAAAGATATCAATGGTAGCT 3′ | shr |
| ZEDHS | 5′ CCCCCATGGATGACGCCTAATAAAGAAGATTACTTG 3′ | mtsR |
| ZEDHA | 5′ AAGGGCTGTGACATAAAGTTGCTTAGC 3′ | mtsR |
| mtsB F | 5′ GCCAGCCATTTTGAGCTCTTTCA 3′ | mtsB |
| dtxR-R | 5′ GGGGAATTCAGCATAGCCTTTGTC 3′ | mtsR |
| dtxR-F | 5′ GGAAGAATTCTTATGGCGACAAGGAACTTG 3′ | mtsR |
| mutF | 5′ GGGAAACTCGAGCTTCCCAACAATCT 3′ | SPy0444 |
| Kan BB S | 5′ AACTAGCTAGCCGGGGGATCAGCTTTTTA 3′ | pBBS2 |
| Kan BB A | 5′ ATACGGAATTCCTTCCAACTGATCTGCGC 3′ | pBBS2 |
| DtxRLKOS | 5′ CCCGAATTCAATTGGAGAACAAGAACCCA 3′ | mtsR |