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Comparison of VirB8 production by the wild-type (wt) strain and mutants for regulator coding genes. The vjbR mutant is used as a negative control (14a). There are 4 × 108 bacteria per well. VirB8 production was evaluated in total cell lysates by Western blotting with VirB8-specific antiserum. The anti-Omp89 antibody was used for the loading control (upper panel).
