TABLE 2.
Virulence of the mutants in BALB/c mice and in cellular modelsa
| Strain | Log CFU
|
||
|---|---|---|---|
| Mice | HeLa | J774 | |
| arsR6 | 2.2 ± 0.1 | 1.9 ± 0.2 | 2.2 ± 0.2 |
| gntR1 | 2.0 ± 0.4 | −0.6 ± 0.1 | −0.7 ± 0.1 |
| gntR2 | 1.0 ± 0.1 | −0.1 ± 0.1 | −0.1 ± 0.1 |
| gntR4 | 0.9 ± 0.1 | 0.5 ± 0.1 | 0.5 ± 0.2 |
| gntR5 | 0.9 ± 0.1 | −0.3 ± 0.1 | −0.6 ± 0.3 |
| gntR10 | 1.2 ± 0.1 | 0.9 ± 0.1 | 0.1 ± 0.1 |
| gntR17 | 0.8 ± 0.2 | 0.3 ± 0.1 | −0.1 ± 0.2 |
| lysR12 | 1.3 ± 0.1 | 0.4 ± 0.2 | −0.1 ± 0.1 |
| lysR13 | 0.8 ± 0.2 | 0.5 ± 0.2 | 0.3 ± 0.2 |
| lysR18 | 1.2 ± 0.1 | 0.7 ± 0.1 | 0.1 ± 0.1 |
| vjbR | ND | 2.0 ± 0.1 | 1.8 ± 0.2 |
a
Virulence is expressed as the log of attenuation of the mutant compared to the wild-type strain. The log of attenuation is defined as the log CFU obtained for the wild-type strain minus the log CFU obtained for mutant strain in the same experiment. The vjbR mutant was used as a control of attenuation in cellular models of infection (14a), i.e., HeLa cells and J774 macrophages. ND, not determined.