FIG. 4.

Ectopic expression of the GGDEF or EAL protein affects ctxA transcription. Strains with the indicated plasmids were grown in MOPS plus NRES with or without 0.1% arabinose to an OD₆₀₀ of 0.6, RNA was isolated, and the ctxA and rpoB transcripts were detected by RPA. L indicates RNA ladder; P indicates undigested probes (only 0.1% of probe used in RPAs was loaded). All samples were normalized to the rpoB loading control and are the average for three independent experiments.