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TcpP and TcpA protein levels are not affected by vieA mutations. A. TcpP was detected by immunoblotting for strains AC-V61 (wild type), AC-V1386 (ΔvieA), AC-V1596 [vieA(E170A)], and AC-V449 (ΔtcpP) grown to an OD600 of 0.6 in M9 + NRES at 30°C. B. TcpA was detected by immunoblotting for strains AC-V61 (wild type), AC-V1386 (ΔvieA), AC-V1596 [vieA(E170A)], and AC-V744 (ΔtoxR) grown to an OD600 of 0.6 in M9 + NRES at 30°C.
