2. Common DESI MSI Setting Ranges Found in the Literature.
| Setting | Value range | |
|---|---|---|
| Distances | ||
| Sprayer – sample | 1–5 mm ,– | |
| Sample – capillary to MS inlet | 0–4 mm , | |
| Angles | ||
| Sprayer – sample | 50°–80° ,,,, | |
| Sample – capillary to MS inlet | 5°–10° | |
| Temperature of capillary to MS inlet | 450–500 °C , | |
| Diameter of capillary to MS inlet | 500 μm , | |
| Solvent | –MeOH/H2O (50:50–98:2) for lipids , | |
| –ACN/H2O (80:20) for proteins | ||
| Acidic/basic additives are often added to boost ionization efficiency and adduct formation. | ||
| Solvent flow rate | 0.5–5 μL/min ,,,,, | |
| Voltage | 4–5 kV , | |
| Gas pressure | 4.5–7 bar | |