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Activation of Rap1B. Platelets were incubated for 5 min with ADP (20 μM), GST-EphB1 (10 μg/ml), or GST-ΔEphB1(10 μg/ml), after which Rap1B in the GTP-bound state was precipitated with GST-RBD and detected with an anti-Rap1 antibody. The results shown are representative of three such experiments.
