Figure 5.

L1/Ng-CAM is expressed in human platelets and forms complexes with EphA4 during platelet aggregation. (A) Western blot of total cell lysates from human brain, endothelial cells (HUVEC) and platelets showing the presence of L1/Ng-CAM. (B) Fluorescence-activated cell sorter analysis of human platelets with an antibody directed against the L1 exodomain. (C) Precipitation of L1 with EphA4 by using an anti-EphA4 antibody. The lysates were prepared from platelets incubated with 20 μM ADP under aggregating conditions in the presence or absence of His-EphA4 plus His-EphB1 (10 μg/ml of each). IP, the immunoprecipitating antibody; IB, the antibody used in the Western immunoblot.