Figure 5.

PRKX activates renal epithelial cell migration. pFLAG/PRKX-, pEGFP-C3-, pFC-PKA-transfected, and control FIB4 cells were labeled with calcein acetoxymethyl ester and plated 24 h posttransfection in Fluoroblock cell migration chamber wells. Four hours postattachment, cells cultured in 1% FBS were either untreated (C) or treated with 8-Br-cAMP (A) or the PKA inhibitor H89 (B) and cultured in wells containing 5% FBS in the lower chamber. The number of migrated cells was determined by fluorescence reading after 0, 4, 8, 12, 18, and 24 h. The values are mean ± SEM from three independent experiments in triplicate.