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. 2002 Jun 24;99(14):9260–9265. doi: 10.1073/pnas.132051799

Figure 6.

Figure 6

PRKX activates epithelial branching morphogenesis of cultured MDCK cells in collagen gels. MDCK cells (A–C) or MDCK cells transfected with an empty expression vector pEGFP-C3 (D–F), PKA expression vector pFC-PKA (G–I), pFLAG/PRKX (J–L), or the kinase-dead pFLAG/PRKX/K78R (M–O) were cultured in type I collagen gels for 10 days. The gels were untreated (A, D, G, J, and M) or treated with 100 μM 8-Br-cAMP (B, E, H, K, and N) or 100 μM 8-Br-cAMP and 10 μM H89 inhibitor (C, F, I, L, and O) and stained with rhodamine-phalloidin for 8 h for fluorescence microscopy visualization.