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. 2025 Jul 31;58(7):288–292. doi: 10.5483/BMBRep.2024-0102

Fig. 3.

Fig. 3

Cytosine base editing by A3A/Y130F-V01 and A3A/Y130F-V04 in protoplasts of A. thaliana and G. max. (A) Structure of the A3A/Y130F-V04 cytosine base editor is illustrated. CBE, cytosine base editor; UGI, uracil DNA glycosylase inhibitor; nCas9, Cas9 nickase; Cde, cytidine deaminase; PAM, protospacer adjacent motif; gRNA, guide RNA; A3A, APOBEC3A; NLS, nuclear localization sequence; T2A, Thosea asigna virus 2A; MCP, MS2 coat protein. (B) C-to-T editing efficiencies of A3A/Y130F-V01 and A3A/Y130F-V04 at three target sites in Arabidopsis protoplasts are presented. (C) Editing windows of A3A/Y130F-V01 and A3A/Y130F-V04 at three target sites in A. thaliana protoplasts are displayed. (D) C-to-T editing efficiencies of A3A/Y130F-V01 and A3A/Y130F-V04 at two independent target sites in G. max protoplasts are shown. (E) Base editing windows of A3A/Y130F-V01 and A3A/Y130F-V04 within two target sites in G. max protoplasts are depicted. In (B, D), each dot signifies a biological replicate. In (B to E), data are rendered as means ± SEM. Distinct letters indicate a significant difference at P < 0.05 (one-way ANOVA with Tukey’s post hoc test).