TABLE 1.
Quantitative analysis of the effect of cell suspension density on chromosome dispersion.
| Tubes | Cell suspension proportion (%) | Cell density (cells/mL) | Suspension turbidity (McF) | Metaphase dispersion area (μm2) | Metaphase number (per slide) | Crossover and overlap count (per metaphase) |
|---|---|---|---|---|---|---|
| #1 | 100 | 2.10 × 107 | 3.74 | 1,445 ± 331a | 13,708 | 1 [1,3]e |
| #2 | 50 | 1.04 × 107 | 1.89 | 1,710 ± 410b | 9,587 | 2 [0,3]e |
| #3 | 25 | 3.49 × 106 | 0.97 | 2,088 ± 462c | 4,771 | 1 [0,2]e |
| #4 | 12.5 | 1.92 × 106 | 0.45 | 2,232 ± 486c | 2,622 | 0 [0,1] |
| #5 | 6.23 | 1.04 × 106 | 0.21 | 2,558 ± 510d | 1,286 | 0 [0,1] |
| #6 | 3.125 | 4.52 × 105 | 0.10 | 2,425 ± 646d | 509 | 0 [0,1] |
| #7 | 1.5625 | 2.87 × 105 | 0.06 | 2,101 ± 475c | 235 | 0 [0,1] |
| #8 | 0.78125 | 9.97 × 104 | 0.02 | 1,985 ± 468c | 122 | 1 [0,1]e |
Notes: a, b, c, and d indicate no significant differences within the same group according to one-way ANOVA, but there are significant differences between different groups; e indicates significant differences compared to the sample in tube #5. The chromosome crossover and overlap counts, which follow a non-normal distribution, are represented by quartiles, i.e., median [25%, 75%].