TABLE 2.
Validation results of the optimal density for chromosome dispersion.
| Samples | Original turbidity (McF) | Metaphase number (per slide) | Original dispersion area (μm2) | Fixative added (mL) | Adjusted metaphase number (per slide) | Adjusted dispersion area (μm2) |
|---|---|---|---|---|---|---|
| 1 | 0.27 | 709 | 2,511 ± 992 | 0.14 | 661 | 2,533 ± 696 |
| 2 | 0.36 | 917 | 2,279 ± 819 | 0.36 | 601 | 2,595 ± 840 |
| 3 | 0.9 | 2,269 | 2,036 ± 455 | 1.64 | 636 | 2,478 ± 714 |
| 4 | 1.14 | 2,926 | 1,930 ± 520 | 2.21 | 672 | 2,494 ± 880 |
| 5 | 0.89 | 2,197 | 2,014 ± 770 | 1.62 | 600 | 2,414 ± 982 |
| 6 | 0.72 | 1,784 | 2,107 ± 740 | 1.21 | 643 | 2,517 ± 715 |
| 7 | 1.13 | 2,907 | 2,080 ± 749 | 2.19 | 674 | 2,413 ± 976 |
| 8 | 1.08 | 2,730 | 2,079 ± 817 | 2.07 | 670 | 2,503 ± 831 |
| 9 | 0.74 | 1,855 | 2,121 ± 649 | 1.21 | 693 | 2,382 ± 581 |
| 10 | 0.45 | 1,176 | 2,448 ± 668 | 0.57 | 619 | 2,459 ± 642 |