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. 2002 Jul 1;99(14):9334–9339. doi: 10.1073/pnas.142627899

Figure 4.

Figure 4

Synthetic lethality of asf1Δbdf1Δ double-knockout strain in mitotic growth. The synthetic lethality of asf1Δbdf1Δ is suppressed by a single-copy plasmid, pRS-ASF1, which expresses the ASF1 gene from its own promoter (pRS-ASF1/asf1Δbdf1Δ, −5-FOA). The viability of this strain without pRS-ASF1 was tested by the addition of 5-FOA into medium (+5-FOA). The pRS316/bdf1Δ strain, the ASF1 gene of which is coded on its chromosome, serves as a control to indicate that the bdf1Δ strain itself is viable in the presence of 5-FOA.