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. 2002 Jul 1;99(14):9358–9363. doi: 10.1073/pnas.142308199

Figure 3.

Figure 3

(A) Recognition of IGR-Heu tumor cell line by autologous T cell clones. IGR-Heu cells (30,000 cells/well) were incubated with Heu127, Heu171, H2307, or H23018 T cells (3,000 cells/well). After 24 h, the amount of TNFα produced by the clones was measured by testing the killing activity of the culture supernatant on the TNF-sensitive WEHI-164c13 cells. (B) Recognition of the peptide-pulsed IGR-Heu cell line by the autologous H23018 clone. IGR-Heu cells were incubated for 20 min at RT with the indicated concentrations of mutated α-actinin-4 peptide before addition of H23018 lymphocytes. After 24 h, the amount of TNFα was measured as in A.