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Binding of HLA tetramers to mutated α-actinin-4-specific T cell clones. Clones were stained with serial concentrations of HLA-A2/α-actinin-4 multimers starting from 2 × 10−2 to 2 × 102 nM under the indicated time and temperature conditions. The cells were washed, fixed with paraformaldehyde, and analyzed by flow cytometry. Data are shown as relative mean fluorescence.
