Figure 1.

Stimulation through the TCR is sufficient for induction of PCKθ capping. Naive AD10 TCR transgenic CD4⁺ T cells were stimulated at 37°C for 45 min with beads coated with anti-CD3 antibodies. As controls, T cells were also incubated with uncoated beads. Cells were stained with anti-PKCθ (A) or phalloidin (B) and analyzed by confocal microscopy. Both PKCθ and phalloidin were localized to the contact region between T cells and the anti-CD3-coated beads. Data are from one representative experiment of five performed.