Figure 4.

Effects of UCP2 overexpression on NO production and NOS II expression. (A) Cells were stimulated with LPS for 48 h, and accumulation of nitrite in the supernatants was measured with Griess reagent. (B) Cells were stimulated with LPS for 0, 6, and 24 h. Amount of NOS II protein was analyzed by Western blotting analysis. (C) Cells were stimulated with LPS for 0, 6, and 24 h. NOS II mRNA expression was analyzed by RT-PCR analysis. (D) Cells were transiently transfected with luciferase reporter construct containing NOS II promoter. After LPS stimulation for 24 h, luciferase activities were determined. (A and D) Results are expressed as the mean ± SEM from quadruplicate cultures.