Skip to main content
. 2002 Jun 21;99(14):9415–9420. doi: 10.1073/pnas.142302399

Figure 2.

Figure 2

In ELISPOT assays, the gp100209–217 epitope-specific T cells recognize PCI-13/IL-2/MEL DNA (recipient cells transduced with DNA derived from gp100+ Mel 526) but not the nontransduced PCI-13/IL-2 (A). The negative controls (no T cells or T cells alone) had a very low background (A). In B, positive and additional negative controls from the same experiment are shown. Note that gp100-negative melanoma (SLM-2) is not recognized, whereas gp100+ Mel 526, the target for these T cells, induced a large number of spots. HLA-A2 or HLA-DR Abs were used to block the MHC-restricted responses in ELISPOT assays, as described in Materials and Methods. The results were obtained in the same ELISPOT assay for IFN-γ production. A representative experiment of four performed is shown. The results in this and all other ELISPOT assays shown are means ± SD from quadruplicate wells.