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PCI-13/IL-2 cells infected with recombinant adenovirus carrying a gene for gp100 express the gp100 epitope and are able to present it to gp100-specific T cells. The results are from a representative ELISPOT assay of three performed. PCI-13/IL-2 cells pulsed with the gp100 peptide or PCI-13/IL-2 infected with adenovirus modified with the EGFP gene did not stimulate the T cells. The asterisk indicates a significant increase in the numbers of spots over controls (P < 0.0001).
