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. 2002 Jun 21;99(14):9415–9420. doi: 10.1073/pnas.142302399

Figure 5.

Figure 5

In A, PCI-13/IL-2 cells transduced by the ballistomagnetic method with DNA obtained from the EGFP+/Mel 562 cells. Twenty-four to 48 h after transduction, 2% of PCI-13/IL-2 cells were fluorescent (Inset shows one such cell, ×400). After enrichment by magnetic selection, apoptosis was induced by UVB irradiation in recipient cells containing Mel DNA/EGFP, and ATC were internalized and processed by HLA-A2+ DC. In B, these DC were used as stimulators in ELISPOT assays. The responders were gp100 epitope-specific T cells. DC-processed PCI-13/IL-2/Mel DNA, but not DC-processed PCI-13/IL-2, induced MHC class I-restricted responses in these T cells. The gp100-specific response was significantly inhibited (*, P = 0.01) by HLA-A2 and w6.32 Abs. A representative experiment of three performed is shown.