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. 2002 Jun 27;99(14):9439–9444. doi: 10.1073/pnas.152313999

Figure 1.

Figure 1

Specific proteins coimmunoprecipitate with intracellular Aβ. Lysates of adult wild-type, CL2006 (Aβ-expressing), or CL2179 (GFP-expressing) animals were incubated with mAb 4G8 or control anti-GFP mAb, and the resulting immunoprecipitates were fractionated on SDS 4–20% polyacrylamide gels and visualized by silver staining. Lane 1, wild-type animals immunoprecipitated with 4G8; lane 2, CL2006 animals immunoprecipitated with 4G8; lane 3, CL2006 animals immunoprecipitated with anti-GFP; lane 4, CL2006 animals immunoprecipitated with 4G8; lane 5, heat-shocked CL2179 animals immunoprecipitated with anti-GFP; lane 6, untreated CL2179 animals immunoprecipitated with anti-GFP. Specific bands reproducibly identified in all CL2006 immunoprecipitation performed to date (nine independent immunoprecipitations) are indicated with asterisks (lanes 2 and 4). All visible bands in lane 2 were subjected to mass spectrometry analysis. The boxes in lane 2 indicate faint bands also recovered for MS analysis. The heavy GFP band in lanes 5 and 6 was confirmed by immunoblot (data not shown). IgG HC, Ig heavy chains; IgG LC, Ig light chains.