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. 2021 Sep 22;35(10):e21939. doi: 10.1096/fj.202002713RR

FIGURE 2.

FIGURE 2

Tissue and molecular response of Creld2eGFP/eGFP mice to a high‐fat diet. (A) Experimental setup. (B) Bodyweight of male mice fed with CD (control diet; n = 13–14) and HFD (high‐fat diet; n = 12–31) for 12 weeks, or HFD for 8 weeks following CD for 4 weeks (n = 14–19). (C) Analysis of insulin resistance induction by HFD via HOMA‐IR (homeostatic model assessment of insulin resistance, CD start: n = 22–29; CD 8 weeks: n = 7–10; HDF 8 weeks: n = 17–20). One‐way ANOVA with Tukey post‐hoc test. (D) Lipid mass spectrometry of livers (n = 5 per condition). DAG: Diacylglycerol. TAG: Triacylglycerol. Circles represent individual mice. One‐way ANOVA. (E) Co‐expression network analysis (CoCena) performed on RNA‐seq data of livers (n = 5 per condition). Numbers indicate genes belonging to a module. Colors represent cluster names: pink: 557 genes, Indian red: 64 genes, dark green: 558 genes, dark gray: 73 genes, orchid: 65 genes, maroon: 141 genes, dark orange: 87 genes, steel blue: 459 genes, gold: 721 genes. GFC: group fold change. (F) Heatmap representation of selected genes from (E), values are displayed as z scores. (G) Lipid mass spectrometry analysis for ceramides. Circles represent individual mice. One‐way ANOVA with Tukey post‐hoc test. (H) Western blot analysis for cleaved Caspase 3 (n = 5). Circles represent individual mice. Unpaired two‐tailed t‐test. *p < .05, **p < .01, ***p < .001, ****p < .0001