FIGURE 6.

Creld2^eGFP/eGFP males develop liver steatosis with progressing age. (A) Thin‐layer chromatography of total lipid extracts from 1‐year‐old animals (♂; n = 4, ♀; n = 3–5). Circles represent individual mice; gray lines are means of control littermates. Unpaired two‐tailed t‐test. (B) Histological analysis of 1‐year‐old male livers by hematoxylin and eosin (HE) and Oil‐red‐O. Scale bars overview: 100 µm. Scale bars insets: 10 µm. (C) qRT‐PCR analysis of male livers (n = 3–5). Gene expression is displayed as relative value. Unpaired two‐tailed t‐test. *p < .05, **p < .01. (D) Protein expression in livers of male mice as assessed by western blot quantification (n = 17–19). For Perk and eIF2α ratios of phosphorylated (P‐Perk and P‐eIF2α) and unphosphorylated protein abundance are displayed. Unpaired two‐tailed t‐test. (E) Flow cytometry analysis of liver Kupffer cells (CD45⁺CD11b^lowF4/80⁺Tim4⁺) in male mice (n = 3–9). (F) qRT‐PCR analysis of pro‐inflammatory cytokines Tnfa, Il1b, and Il6 in bone‐marrow‐derived macrophages after LPS treatment isolated from males (n = 3–4). Error bars represent ± SD