Figure 3.

Rapid and transient attachment of VE-cadherin and VEGFR2 to the endothelial cytoskeleton in endothelial cells exposed to shear stress. BAECs were grown under static conditions or exposed to LSS (10 dynes/cm²) for various time intervals. Cytoskeleton-free (Triton X-100-soluble) and bound (Triton X-100-insoluble) fractions were prepared and tested for the presence of VE-cadherin (A), β-catenin (B), and VEGFR2 (C) by using immunoblotting. The data shown is a representative experiment (n = 5). ST, static BAEC control cultures.