Figure 4.
Rapid and transient attachment of VE-cadherin to β-catenin and VEGFR2 in endothelial cells exposed to shear stress. BAECs were grown under static conditions or exposed to LSS (10 dynes/cm2) for various time intervals. (A) Coimmunoprecipitation (IP) was performed to whole-cell extracts by using an antibody to β-catenin. Precipitated samples were tested for the presence of VE-cadherin (VE-cad) or β-catenin by using immunoblotting (IB). (B) Coimmunoprecipitation was performed with whole-cell extracts by using an antibody to VEGFR2. Precipitated samples were tested for the presence of VE-cadherin or VEGFR2 by using immunoblotting. ST, static BAEC control cultures.