Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Saccharomyces cerevisiae) | W303 | Lab stock | G16 | Strains are listed in Supplementary file 1, table 1 |
| Sequence-based reagent | gRNA sequence | IDT | N/A | Sequences are listed in Supplementary file 1, table 2 |
| Chemical compound, drug | Ni-NTA Agarose | QIAGEN | Cat# 30210 | |
| Chemical compound, drug | (S)- (+)-Camptothecin (CPT) | Sigma | Cat# C9911 | |
| Antibody | F9 anti-phosphorylated Rad53 (mouse polyclonal) | Pellicioli et al., 2001 | N/A | WB: 1:250 |
| Antibody | Anti-Rad9 (rabbit polyclonal) | Usui et al., 2009 | N/A | WB: 1:3000 |
| Antibody | Anti-Mec1-S1964-p (rabbit polyclonal) | Memisoglu et al., 2019 | N/A | WB: 1:500 |
| Antibody | Anti-Srs2 (goat polyclonal) | Santa Cruz (yC-18) | Cat# sc-11991; RRID:AB_672740 |
WB: 1:1000 |
| Antibody | Anti-Pgk1 (mouse monoclonal) | Invitrogen (22C5D8) | Cat# 459250; RRID:AB_2532235 |
WB: 1:8000 |
| Antibody | Anti-Myc | Thermo Fisher (9E10) | Cat# 13-2500; RRID:AB_2533008 |
IP: 1 μg/ml |
| Peptide, recombinant protein | α factor | GenScript | Cat# RP01002 | |
| Peptide, recombinant protein | Pronase protease | Millipore | Cat# 53702 | |
| Commercial assay or kit | 4–20% precast polyacrylamide gel | Bio-Rad | Cat# 4561096 | |
| Commercial assay or kit | NuPAGE Tris-Acetate Mini Protein Gels, 3–8%, 1.0–1.5 mm | Thermo Fisher Invitrogen | Cat# EA03752 | |
| Commercial assay or kit | NuPAGE Tris-Acetate Mini Protein Gels, 7%, 1.0–1.5 mm | Thermo Fisher Invitrogen | Cat# EA03555 | |
| Software, algorithm | Prism 9.0 | GraphPad | RRID:SCR_002798 | |
| Software, algorithm | LAS-3000 imaging system | Fuji | N/A | |
| Software, algorithm | ImageJ | FiJi | RRID:SCR_002285 |