| Strain, strain background (Mus musculus, male) |
DATIREScre (B6.SJL-Slc6a3tm1.1(cre)Bkmn/J) |
Jackson Laboratory |
IMSR_JAX: 000664 |
|
| Strain, strain background (M. musculus, male) |
C57BL/6 J |
Charles River Laboratories |
Strain code 631 |
|
| Strain, strain background (AAV) |
AAV5-EF1α-DIO-ChR2-eYFP |
University of North Carolina Vector Core |
|
https://www.med.unc.edu/genetherapy/vectorcore/in-stock-aav-vectors/deisseroth/
|
| Strain, strain background (AAV) |
AAV5-EF1α-DIO-eYFP |
University of North Carolina Vector Core |
|
https://www.med.unc.edu/genetherapy/vectorcore/in-stock-aav-vectors/deisseroth/
|
| Strain, strain background (AAV) |
AAV9-Syn-ChrimsonR-tdTomato |
University of North Carolina Vector Core |
|
https://www.med.unc.edu/genetherapy/vectorcore/in-stock-aav-vectors/deisseroth/
|
| Strain, strain background (AAV) |
HSV-LS1L-mCherry-IRES-flpo |
Viral Gene Transfer Core Facility at MIT |
|
https://biology.mit.edu/faculty-and-research/core-facilities/
|
| Strain, strain background (AAV) |
AAV5-EF1α-DIO-eNpHR3.0-eYFP |
Addgene |
RRID:Addgene_26966
|
|
| Strain, strain background (AAV) |
AAV1-syn-jGCaMP7f |
Addgene |
RRID:Addgene_104488
|
|
| Strain, strain background (AAV) |
AAV1-CAG-TdTomato |
UPenn vector core |
|
https://www.med.upenn.edu/carot/aav-core.html
|
| Chemical compound |
CTB-555 |
ThermoFisher |
C34776 |
|
| Chemical compound |
CTB-647 |
ThermoFisher |
C34778 |
|
| Chemical compound |
DAPI |
Sigma-Aldrich |
D9542 |
1:50,000 |
| Chemical compound |
PVA-DABCO |
Sigma-Aldrich |
10981 |
|
| Chemical compound |
Normal Donkey Serum |
Jackson Immunoresearch |
RRID:AB_2337258
|
|
| Chemical compound |
CF405-conjugated streptavidin |
Biotium |
29032 |
1:1000 |
| Chemical compound, drug |
CF633-conjugated streptavidin |
Biotium |
29037 |
1:1000 |
| Chemical compound |
RNAScope Multiplex Fluorescent Reagent Kit V2 |
ACDBio |
323110 |
|
| Chemical compound |
RNAScope Multiplex Detection Reagents |
ACDBio |
323110 |
|
| Chemical compound |
RNAScope Drd1a target probe |
ACDBio |
406491-C1 |
|
| Chemical compound |
RNAScope Drd2 target probe |
ACDBio |
406501-C3 |
|
| Chemical compound |
RNAScope Npbwr1 target probe |
ACDBio |
547181-C1 |
|
| Chemical compound |
RNAScope Vipr2 target probe |
ACDBio |
465391-C1 |
|
| Chemical compound |
TSA fluorophore (green) |
PerkinElmer |
Fluorescein |
|
| Chemical compound |
TSA fluorophore (red) |
PerkinElmer |
Cyanine 3 |
|
| Chemical compound |
TSA fluorophore (far red) |
PerkinElmer |
Cyanine 5 |
|
| Antibody |
Anti-Tyrosine Hydroxylase Antibody |
Millipore |
RRID:AB_570923
|
1:1000 |
| Antibody |
Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) |
Jackson ImmunoResearch |
RRID:AB_2340375
|
1:1000 |
| Antibody |
Alexa Fluor 647-AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) |
Jackson ImmunoResearch |
RRID:AB_2340379
|
1:1000 |
| Software |
Fluoview software version 4.0 |
Olympus |
RRID:SCR_014215
|
|
| Software |
FIJI |
ImageJ |
RRID:SCR_003070
|
|
| Software |
CellProfiler |
CellProfiler |
RRID:SCR_007358
|
|
| Software |
Adobe Photoshop CC |
Adobe |
RRID:SCR_014199
|
|
| Software |
MATLAB |
Mathworks |
RRID:SCR_001622
|
|
| Software |
Adobe Illustrator |
Adobe |
RRID:SCR_010279
|
|
| Software |
Ethovision XT |
Noldus |
RRID:SCR_000441
|
|
| Software |
pClamp 10.4 software |
Molecular Devices |
RRID:SCR_011323
|
|
| Software |
GraphPad Prism 8 |
Graphpad |
RRID:SCR_002798
|
|
| Other |
Optic fiber |
Thor Labs |
TS1843490 |
|
| Other |
Ferrules |
Kientec Systems |
FSS-LC-330 |
|
| Other |
Small animal stereotaxic frame |
David Kopf Instruments |
Model 942 |
|
| Other |
0.10 mL Microsyringe |
World Precision Instruments (NANOFIL-NF33BL-2) |
RRID:SCR_008593
|
|
| Other |
Microsyringe Pump UMP3 and Controller Micro4 |
World Precision Instruments (UMP3-3) |
RRID:SCR_008593
|
|
| Other |
Peristaltic pump for ex vivo recordings |
Minipuls 3 Gilson |
F155001 |
|
| Other |
Pulse generator |
A.M.P.I. Master-8 |
RRID:SCR_018889
|
|
| Other |
Cryostat |
Leica biosystems CM3050 S |
RRID:SCR_020214
|
|
| Other |
HM430 Microtome |
Thermo Fisher Scientific |
RRID:SCR_020020
|
|
| Other |
Vibrating blade vibratome |
Leica Biosystems VT1200 |
RRID:SCR_018453
|
|
| Other |
Confocal Laser-Scanning microscope |
Olympus FV1000 |
RRID:SCR_020337
|
|
| Other |
Diode Blue 473 nm Laser |
OptoEngine LLC |
MBL-III-473/1–100 mW |
|
| Other |
Horizontal puller for glass microelectrodes for ex vivo recordings |
Sutter P-1000 |
RRID:SCR_021042
|
|
| Other |
Multiclamp amplifier for ex vivo recordings |
Molecular Devices 700B |
RRID:SCR_018455
|
|
| Other |
Microscope for ex vivo recordings |
Scientifica |
RRID:SCR_021035
|
|
| Other |
Microscope for in vivo calcium imaging recordings |
Inscopix nVoke System |
RRID:SCR_023028
|
|
