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. 2002 Aug 5;99(17):10970–10975. doi: 10.1073/pnas.172221699

Fig 4.

Fig 4.

Gel (2% agarose) of RT-PCR products showing amplification with P. shumwayae-specific primer. Amplification of the expected 400-bp sequence was observed with cDNA from P. shumwayae (CCMP 2089) as well as the same culture exposed to larval C. variegatus 18 h before RNA extraction using the primers PfBSU/PfBITSR201L (lanes 2 and 5), confirming the presence of P. shumwayae cDNA. No amplification was seen (lane 8) with the algal food, R. salina. Positive amplification was also observed for all positive controls of P. shumwayae genomic DNA (lanes 3, 6, and 9). No amplification was observed for any of the negative controls of no template (lanes 4, 7, and 10). Lanes 1 and 11 are 100-bp DNA ladders.