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. 2002 Aug 12;99(17):11025–11030. doi: 10.1073/pnas.162172899

Fig 5.

Fig 5.

Activated MST1 rescues the caspase 3−/− myoblast phenotype. (A) Kinase activity of MST1 protein constructs. MST1-act-transfected cells display a high level of kinase activity. Accuracy of sample loading is indicated by the Coomassie-stained gel shown below (n = 4). (B) Caspase 3 knockout myoblasts transfected with vector alone failed to differentiate. (C) Caspase 3 knockout myoblasts containing MST1-act differentiated to form fused, multinucleated myotubes. The efficiency of differentiation in MST1-act myoblasts was >85%. Total nuclei were visualized by using DAPI staining (D and E). (F–I) Wild-type myoblasts were transfected with vector or with MST1-act and allowed to differentiate for up to 4 days. After 2 days of differentiation, cells containing MST1-act (H) share a similar phenotype with vector-transfected cultures differentiated for 4 days (G). Extensive cell death is observed when cells are transfected with MST1-act and differentiated for 4 days (I).