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. 2002 Aug 12;99(17):11043–11048. doi: 10.1073/pnas.162375799

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Fig 3. — RP-HPLC/TLC/RIA/MS analysis after sad- or GFP-transfected S2 cell incubation (8 h) with the 2dE substrate (30–100% methanol gradient). Ecdysteroid immunoreactivity was quantified by RIA (1/1000 of total sample) by using the SHO-3 antibody after incubations with sad (filled circles) or GFP (open circles). UV absorption was measured at 248 nm (solid line). (Inset, TLC) TLC/RIA (chloroform/ethanol) of RP-HPLC-purified ecdysone (E) product (1/1000 of total sample). (Inset, MS) RP-HPLC/ESI-MS on an LCQ^DECA (Thermo Finnigan, San Jose, CA) of the TLC-purified E product.