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. 2002 Aug 12;99(17):11043–11048. doi: 10.1073/pnas.162375799

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Copyright © 2002, The National Academy of Sciences

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Fig 4. — RP-HPLC/TLC analysis of ecdysteroids after sad-, dib-, sad and dib-, or GFP-transfected S2 cell incubations with the [³H]ketotriol (2,22dE) substrate. (A) sad (filled circles) or GFP (open circles) + [³H]ketotriol. ?, Unidentified metabolite conjugate. (Inset) TLC analysis of [³H[22dE product. (B) dib (filled circles) or GFP (open circles) + [³H]ketotriol. (Inset) TLC of [³H]2dE product. (C) sad and dib (filled circles) or GFP (open circles) + [³H]ketotriol. (Inset) TLC of [³H]E (ecdysone) product.