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. 2002 Aug 12;99(17):11061–11066. doi: 10.1073/pnas.172197099

Fig 4.

Fig 4.

Short-gap-filling reactions catalyzed by pol V as a function of RecA concentration in the presence and absence of a template abasic lesion. (a) 2-nt DNA template gap; (b) 3-nt DNA template gap; (c) 63-nt ssDNA template overhang, containing an abasic lesion, in the absence and presence of SSB; (d) 3-nt DNA template gap, containing an abasic lesion, in the absence and presence of SSB. ATP-γ-S was present in all reactions. The p/t DNA concentration was 10 nM. The ratio of RecA/DNA nt for each data point was: 25 nM RecA (1 RecA:50 DNA template nt); 250 nM RecA (1:5); 600 nM RecA (1:2). The arrows indicate the location of an abasic template lesion, X. The p/t DNA is shown at the top of each panel.