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. 2002 Aug 2;99(17):11067–11072. doi: 10.1073/pnas.162373299

Fig 2.

Fig 2.

NusA stimulates pausing at U107 and U144 in the trp leader region. In vitro transcription reactions were performed with the NTP concentrations listed at the top. Reactions were carried out in the absence (−) or presence (+) of 1 μM NusA. Transcription reactions were stopped at the times indicated above each lane. Reactions corresponding to “chase” were extended for an additional 5 min in the presence of 500 μM of each NTP. The positions of paused (U107 and U144), terminated (U141 and U142), and run-off (RO) transcripts are shown.