Fig 5.

TRAP binding releases RNA polymerase from NusA-stimulated U107 pause complexes. Pause complexes were allowed to form in the presence of 1 μM NusA for 30 s before the addition of TRAP at the indicated concentration. Reactions were stopped at the times shown at the top of each lane. Note that the time points for the reaction containing 1 μM TRAP differ from the other two reactions. Positions of paused (U107 and U144), terminated (G140, U141, and U142), and run-off (RO) transcripts are shown. Reactions corresponding to “chase” were extended for an additional 5 min in the presence of 500 μM of each NTP. Only fragments of the gel containing bands of interest are shown.