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Effect of antisense oligonucleotides on pausing and termination. Assays contained 0.1 mM oligonucleotide (oligo) B, A, or T complementary to bases 55–69, 70–84, or 106–120 of the trp leader RNA, respectively. Transcription reactions were stopped at the times indicated above each lane. The positions of paused (U107 and U144), terminated (G140, U141, and U142), and run-off (RO) transcripts are shown. Only fragments of the gel containing bands of interest are shown.
