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Expression and surface targeting of the wt, D309A, D309E, Y227F, and Y227A mGlu8 receptors. (a) Western blot analysis of the wild-type and mutant mGlu8a receptor showing the expected molecular mass for their dimeric form. (b) Cells expressing the indicated mGlu8a receptor epitope-tagged at their N-terminal extracellular end were labeled with the HA antibody. Cells were not permeabilized such that only the surface receptor could be detected.
