Fig 2.

Interactions of GhCesA1 and GhCesA2 N-terminal region in yeast and in vitro. (A) Schematic presentation of the GhCesA1 and GhCesA2 N-terminal region (Upper) that contain the Zn domains (black). Yeast transformed with the plasmids containing the GAL-4 binding domain (pGAL^bd: bait) and the GAL-4 activating domain (pGAL^ad: prey), as indicated, were grown on synthetic complete medium minus leu and trp (-LT) and minus leu, trp, and his and 5 mM 3-amino 1,2,4-triazole (-LTH + 3-amino-1,2,4-triazol). β-Galactosidase activity was assayed on –LT medium. (B) Purified recombinant GhCesA1 N-terminal GST fusion (A1N₁₁₅), GhCesA2 N-terminal GST fusion (A2N₁₁₁), and GST (“Input”) incubated with His-tagged GhCesA1 Zn domains (A1_zd) were washed and eluted (“Output”) and proteins subjected to 12% SDS/PAGE developed by silver staining.