Fig 4.
Redox potential regulates dimerization of H-A1zd. (A) H-A1zd pretreated with 10 mM DTT at 4°C for 15 min was incubated for an additional 15 min at 4°C in the presence of increasing concentrations of H2O2 as indicated and analyzed on 15% nondenaturing PAGE developed by silver staining. Untreated protein (30 min at 4°C) served as control. The 14.2-kDa lactalbumin marker is indicated. (B) H-A1zd was incubated in the presence of DTT and diamide as indicated and subjected to 15% SDS/PAGE developed by silver staining.